SeeTrue Microcapillary Needles
SeeTrue needle impacts and advances typical procedures extensively used in biomedical fields. It bridges an important need in microinjection technology and makes this widely used procedure more reliable and easier to perform. More on solving deficiencies of current needles
Microinjection entails using a clear glass microneedle to inject foreign materials (e.g. DNA and RNA) and cells into biological targets such as an individual cell in culture, egg, embryo, or intracellular space. This thirty-year old transgenic engineering technique has driven advances in the fundamental research in cell and systems biology, stem-cell gene manipulation, in vitro fertilization cycle therapy, biomedical transgenic research and allowed extensive human disease prevention modeling via pathophysiological research. Manual microinjection technique requires great practitioner skill, combining physical dexterity with sustained attention to detail.
Improving Microinjection Process
SeeTrue microcapillary needle improves the technical capabilities, reproducibility and effectiveness of an important fundamental microinjection process used in many areas of biomedical sciences.
Increasing Efficiency and Reproducibility
SeeTrue needle has the potential to increase the efficiency and reproducibility of manual microinjection as well as automated visual based robot-controlled injection.
Solving Deficiencies in Using the Current Needle
Visibility - ability to visualize and locate the needle inside the injected cell
Clogging - dramatic reduction of the clogging rate with the SeeTrue needle
Calibration variability - unified needles so calibration time and variability are greatly reduced
Inner-needle modifications that will eliminate adherence of cells into the inner walls of the microcapillary needle
Problems with Using the Current Industry-Standard Microneedles (ISNs)
Industry-Standard Microneedles (ISNs) have four major deficits, or customer pain-points that negatively impact experimental design, execution, repeatability, and overall efficacy:
Calibration variability: manual creation of each needle-tip introduces undesired variability; because each needle tip is calibrated and opened manually, an inherent variability exists in the volume of material that will be injected into the cells from different needles.
Susceptibility to clogging: cytoplasmic material (e.g., embryo yolk) can clog the needle-tip opening.
Low contrast visibility: the ISN’s transparency creates low contrast visibility in vitro, leaving the user unable to track the penetrating needle-tip leading to missed-injection targets.
Adherence: injected cells can adhere to the inner walls of the glass capillary needle leading to costly injected material loss (e.g., sperm, human oocyte). In this case, both the needle and cells within it must be discarded and the process of needle preparation needs to be repeated.